Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7678
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 871701
Product Specifications
Immunogen
E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
Asp2-Tyr323
Accession # P42330
Asp2-Tyr323
Accession # P42330
Specificity
Detects human Aldo-keto Reductase 1C3/AKR1C3 in ELISAs and Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Aldo-keto Reductase 1C3/AKR1C3 in LNCaP Human Cell Line.
Aldo-keto Reductase 1C3/AKR1C3 was detected in immersion fixed LNCaP human prostate cancer cell line stimulated with 50 ng/mL Recombinant Human/Mouse/Rat ActivinA (Catalog # 338-AC) for 24 hrs using Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Simple WesternTM.
Simple Western lane view shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 45 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line stimulated with 50 ng/mL Recombinant Human/Mouse/Rat ActivinA (Catalog # 338-AC) for 24 hrs
Sample: Immersion fixed LNCaP human prostate cancer cell line stimulated with 50 ng/mL Recombinant Human/Mouse/Rat ActivinA (Catalog # 338-AC) for 24 hrs
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Simple Western
5 µg/mL
Sample: A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Western Blot
0.5 µg/mL
Sample: A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB7678 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldo-keto Reductase 1C3/AKR1C3
AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.
Long Name
Aldo-keto Reductase Family 1, Member C3
Alternate Names
AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS
Gene Symbol
AKR1C3
UniProt
Additional Aldo-keto Reductase 1C3/AKR1C3 Products
Product Documents for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Product Specific Notices for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
For research use only
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