Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7678
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
Met1-Tyr323
Accession # P42330
Met1-Tyr323
Accession # P42330
Specificity
Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot.
Western blot shows lysates of Huh-7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Huh‑7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue
Sample: Huh‑7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldo-keto Reductase 1C3/AKR1C3
AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.
Long Name
Aldo-keto Reductase Family 1, Member C3
Alternate Names
AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS
Gene Symbol
AKR1C3
UniProt
Additional Aldo-keto Reductase 1C3/AKR1C3 Products
Product Documents for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
Product Specific Notices for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
For research use only
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