Human Alkaline Phosphatase/ALPL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29091
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 388828
Product Specifications
Immunogen
E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Ser502
Accession # P05186
Leu18-Ser502
Accession # P05186
Specificity
Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100%
cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed
and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP
or rhAlkaline Phosphatase/ALPI is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Alkaline Phosphatase/ALPL Antibody
Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells.
Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Alkaline Phosphatase/ALPL Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 5 using MAB29091 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.
References
- Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
- Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
UniProt
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Human Alkaline Phosphatase/ALPL Antibody
Product Specific Notices for Human Alkaline Phosphatase/ALPL Antibody
For research use only
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