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Human Alkaline Phosphatase/
ALPL Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1448

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Cited:

Cell Selection, Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # B4-78

Product Specifications

Immunogen

Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

Specificity

Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Alkaline Phosphatase/ALPL Biotinylated Antibody

Alkaline Phosphatase/ALPL antibody in Human Osteoblasts by Immunocytochemistry (ICC).

Alkaline Phosphatase/ALPL in Human Osteoblasts.

Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Alkaline Phosphatase/ALPL Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood granulocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated human osteoblasts

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine

Reviewed Applications

Read 1 review rated 4 using BAM1448 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References

  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Gene Symbol

ALPL

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Human Alkaline Phosphatase/ALPL Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Alkaline Phosphatase/ALPL Biotinylated Antibody

For research use only

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