Human Allergin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7364
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Allergin-1 (isoform Allergin-1S2)
Met29-Ser131
Accession # BAJ08253
Met29-Ser131
Accession # BAJ08253
Specificity
Detects human Allergin-1 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Allergin-1 Antibody
Detection of Human Allergin-1 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Allergin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7364) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Allergin-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Allergin-1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human plasma
Sample: Human plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Allergin-1
MCA32 (Mast Cell Antigen; also Allergin-1 and c17orf60) is a 70 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils and macrophages, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilonRI. Upon IgE mediated Fc epsilonRI aggregation and activation, MCA32 recruits Tyr phosphatases to a Fc epsilonRI tetrameric complex, dampening IgE receptor downstream signaling. Mature human MCA32 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length (aa 20-343) (SwissProt #:Q7Z6M3). It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40 kDa splice variants exist that show deletions of the Ig-like domains. One isoform termed MCA32S1 is missing aa 128-217, while a second isoform called MCA32S2 is missing aa 33-122. This second isoform represents the antigen used for immunization in this product (GenBank #:BAJ08253). Over aa 29-131, the Allergin-1S2 isoform shares 100% sequence identity with human Allergin-1L full length isoform and only 24% aa sequence identity with mouse MCA32.
Long Name
Allergy Inhibitory Receptor 1
Alternate Names
Allergin1, C17orf60, MCA32, MCA32-1S2, MILR1
Gene Symbol
MILR1
UniProt
Additional Allergin-1 Products
Product Documents for Human Allergin-1 Antibody
Product Specific Notices for Human Allergin-1 Antibody
For research use only
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