Human alpha-Synuclein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11376
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1060506
Product Specifications
Immunogen
E. coli-derived human alpha-Synuclein
Met1-Ala140
Accession # P37840
Met1-Ala140
Accession # P37840
Specificity
Detects human alpha-Synuclein in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human alpha-Synuclein Antibody
Detection of alpha-Synuclein in SK-Mel-28 cells (positive) and HL-60 cells (negative).
alpha-Synuclein was detected in immersion fixed SK-Mel-28 human malignant melanoma cells (positive) and absent in HL-60 human acute promyelocytic leukemia cells (negative) using Mouse Anti-Human alpha-Synuclein Monoclonal Antibody (Catalog # MAB11376) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of alpha-Synuclein in Human Brain Cerebellum.
alpha-Synuclein was detected in immersion fixed paraffin-embedded sections of Human Brain Cerebellum using Mouse Anti-Human alpha-Synuclein Monoclonal Antibody (Catalog # MAB11376) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to medulla. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human alpha-Synuclein Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and HL‑60 human acute promyelocytic leukemia cells (negative)
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and HL‑60 human acute promyelocytic leukemia cells (negative)
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cerebellum
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cerebellum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Synuclein
alpha-Synuclein is member of a family of small soluble proteins that include also beta-, and gamma-Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where it cycles between a free, partially unfolded and a helical, membrane-bound form. alpha-Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha-Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s Disease, Parkinson’s Disease Dementia, and dementia with Lewy bodies. alpha-Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s Disease.
References
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Breydo L. et al. (2012) Biochim. Biophys. Acta. 1822:261.
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Chen R.H. et al. (2013) J. Biol. Chem. 288:7438.
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Li X. et al. (2008) Acta Biochim. Biophys. Sin (Shanghai) 40:406.
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Surguchov A. (2008) Int. Rev. Cell Mol. Biol. 270:225.
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Xia Q. et al. (2008) Front. Biosci. 13:3850.
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
UniProt
Additional alpha-Synuclein Products
Product Documents for Human alpha-Synuclein Antibody
Product Specific Notices for Human alpha-Synuclein Antibody
For research use only
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