Human alpha-Synuclein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13383
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 970114
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Synuclein
Met1-Ala140
Accession # P37840
Met1-Ala140
Accession # P37840
Specificity
Detects human alpha-Synuclein in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha-Synuclein Antibody
Detection of Human alpha-Synuclein by Western Blot.
Western blot shows lysates of human brain (motor cortex and cerebellum) tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
alpha-Synuclein in Human Brain.
a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human alpha-Synuclein Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
5 µg/mL
Sample: Human brain (motor cortex and cerebellum) tissue
Sample: Human brain (motor cortex and cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB13383 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Synuclein
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
UniProt
Additional alpha-Synuclein Products
Product Documents for Human alpha-Synuclein Antibody
Product Specific Notices for Human alpha-Synuclein Antibody
For research use only
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