Human alpha1-Acid Glycoprotein Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3694
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human plasma-derived alpha1-Acid Glycoprotein
Specificity
Detects human alpha1-Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mose alpha1-Acid Glycoprotein is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human alpha1-Acid Glycoprotein Antibody
Detection of Human alpha1-Acid Glycoprotein by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a1-Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha1‑Acid Glycoprotein by Simple WesternTM.
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for a1-Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human alpha1-Acid Glycoprotein Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human serum
Sample: Human serum
Western Blot
0.1 µg/mL
Sample: Human plasma
Sample: Human plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Gene Symbol
ORM1
Additional alpha 1-Acid Glycoprotein Products
Product Documents for Human alpha1-Acid Glycoprotein Antibody
Product Specific Notices for Human alpha1-Acid Glycoprotein Antibody
For research use only
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