Human alpha1-Microglobulin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7724

R&D Systems, part of Bio-Techne
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MAB7724
MAB7724-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Ovine

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 784917

View all alpha 1-Microglobulin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human alpha1‑Microglobulin
Gly20-Val203
Accession # P02760

Specificity

Detects human alpha1‑Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha1-Microglobulin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha1-Microglobulin Antibody

Detection of Human a1-Microglobulin antibody by Western Blot.

Detection of Human alpha1‑Microglobulin by Western Blot.

Western blot shows lysates of human liver tissue, Hep3B human hepatocellular carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Microglobulin Monoclonal Antibody (Catalog # MAB7724) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a1-Microglobulin at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a1-Microglobulin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

alpha1-Microglobulin in HepG2 Human Cell Line.

a1-Microglobulin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human a1-Microglobulin Monoclonal Antibody (Catalog # MAB7724) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human alpha1-Microglobulin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: Human liver tissue, Hep3B human hepatocellular carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Huh‑7 human hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Microglobulin

Human alpha1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo.  alpha1-Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha1-Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human alpha1-Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha1-Microglobulin protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). alpha1-Microglobulin possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa alpha1-Microglobulin and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact.  alpha1-Microglobulin will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57.  Over aa 20-203, human alpha1-Microglobulin shares 76% aa sequence identity with both mouse and rat alpha1-Microglobulin.

Alternate Names

A1M, alpha 1Microglobulin, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Entrez Gene IDs

259 (Human); 11699 (Mouse); 25377 (Rat)

Gene Symbol

AMBP

UniProt

P02760 (Human)

Additional alpha 1-Microglobulin Products

Product Documents for Human alpha1-Microglobulin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human alpha1-Microglobulin Antibody

For research use only

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