Human Aminopeptidase N/CD13 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3815
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Co-Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 498001
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
Lys69-Lys967
Accession # AAA51719
Lys69-Lys967
Accession # AAA51719
Specificity
Detects human Aminopeptidase N/CD13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Aminopeptidase N is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Aminopeptidase N/CD13 Antibody
Detection of Human Aminopeptidase N/CD13 by Western Blot.
Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Aminopeptidase N/CD13 in SH-SY5Y Human Cell Line.
Aminopeptidase N/CD13 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aminopeptidase N/CD13 in Human Liver Cancer Tissue.
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human Aminopeptidase N/CD13 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue and immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue and immersion fixed paraffin-embedded sections of human brain (cortex)
Knockout Validated
Aminopeptidase N/CD13
is specifically detected in U937 human histiocytic lymphoma parental cell
line but is not detectable in Aminopeptidase N/CD13 knockout U937
human histiocytic lymphoma cell line.
Simple Western
40 µg/mL
Sample: Human small intestine tissue and Human prostate tissue
Sample: Human small intestine tissue and Human prostate tissue
Western Blot
2 µg/mL
Sample: Human kidney tissue and human prostate tissue
Sample: Human kidney tissue and human prostate tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3815 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase N/CD13
The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptidase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).
References
- Olsen, J. et al. (1988) FEBS Lett. 238:307.
- Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
- Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.
Alternate Names
Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN
Gene Symbol
ANPEP
UniProt
Additional Aminopeptidase N/CD13 Products
Product Documents for Human Aminopeptidase N/CD13 Antibody
Product Specific Notices for Human Aminopeptidase N/CD13 Antibody
For research use only
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