Human Aminopeptidase O/ONPEP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4647
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 562912
Product Summary for Human Aminopeptidase O/ONPEP Antibody
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase O/ONPEP
Met1-Phe819
Accession # Q8N6M6
Met1-Phe819
Accession # Q8N6M6
Specificity
Detects human Aminopeptidase O/ONPEP in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Aminopeptidase O/ONPEP Antibody
Aminopeptidase O/ONPEP in Human Kidney.
Aminopeptidase O/ONPEP was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Aminopeptidase O/ONPEP Monoclonal Antibody (Catalog # MAB4647) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Aminopeptidase O/ONPEP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and human kidney cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human kidney and human kidney cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase O/ONPEP
Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N terminal Arg and Asn residues. Human Aminopeptidase O is 819 amino acids in length. It contains one catalytic domain (aa 167‑589) with a Zn-binding site (His479-His483) and a C terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455‑553; a second has an 11 aa substitution for the C terminal 265 amino acids; and a third shows both a deletion of aa 455‑553 and an 11 aa substitution for the C-terminal 46 amino acids. Full length human Aminopeptidase O is 76% aa identical to mouse Aminopeptidase O.
Alternate Names
APO, C90RF3, C9orf3, ONPEP
Gene Symbol
AOPEP
UniProt
Additional Aminopeptidase O/ONPEP Products
Product Documents for Human Aminopeptidase O/ONPEP Antibody
Product Specific Notices for Human Aminopeptidase O/ONPEP Antibody
For research use only
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