Human Aminopeptidase P2/XPNPEP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB24901
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 391131
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase P2/XPNPEP2
His22-Ala650
Accession # AAB96394
His22-Ala650
Accession # AAB96394
Specificity
Detects human Aminopeptidase P2/XPNPEP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human XPNEP1 or recombinant mouse XPNEP2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Aminopeptidase P2/XPNPEP2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN)
Sample: Recombinant Human Aminopeptidase P2/XPNPEP2 (Catalog # 2490-ZN)
Neutralization
Measured by its ability to neutralize Recombinant Human Aminopeptidase P2/XPNPEP2 (2 µg/mL, Catalog # 2490‑ZN) cleavage of the fluorogenic peptide substrate Lys(Abz)-PP-pNA (0.05 mM). The Neutralization Dose (ND50) is typically 1.5 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase P2/XPNPEP2
The human XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1‑4). It is a member of the M24 family of metalloproteases, which also contains methionine Aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (5). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (6). Human APP2 is widely expressed in many adult tissues with the highest levels in the kidney (7).
References
- Venema, R.C. et al. (1997) Biochim. Biophys. Acta 1354:45.
- Sprinkle, T.J. et al. (1998) Genomics 50:114.
- Cottrell, G.S. et al. (1998) Biochem. Soc. Trans. 26:S248.
- Prueitt, R.L. et al. (2000) Cytogenet. Cell Genet. 89:44.
- Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
- Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 934, Elsevier Academic Press, San Diego.
- Ersahin C. et al. (2005) Arch. Biochem. Biophys. 435:303.
Alternate Names
XPNPEP2
Gene Symbol
XPNPEP2
UniProt
Additional Aminopeptidase P2/XPNPEP2 Products
Product Documents for Human Aminopeptidase P2/XPNPEP2 Antibody
Product Specific Notices for Human Aminopeptidase P2/XPNPEP2 Antibody
The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718. Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.
For research use only
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