Human Amphiphysin/AMPH Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6527

R&D Systems, part of Bio-Techne
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MAB6527
MAB6527-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 694145

View all Amphiphysin/AMPH Antibodies »

Product Summary for Human Amphiphysin/AMPH Antibody

Immunogen

E. coli-derived recombinant human Amphiphysin/AMPH
Asn204-Gly300
Accession # P49418

Specificity

Detects human Amphiphysin/AMPH in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Amphiphysin/AMPH Antibody

Amphiphysin/AMPH antibody in Human Brain by Immunohistochemistry (IHC-P).

Amphiphysin/AMPH in Human Brain.

Amphiphysin/AMPH was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Amphiphysin/AMPH Monoclonal Antibody (Catalog # MAB6527) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Amphiphysin/AMPH Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB6527 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiphysin/AMPH

AMPH (Amphiphysin; also AMPH1) is a member of the Amphiphysin family, BAR superfamily of proteins.  Although its calculated MW is only 78 kDa, it runs anomalously in SDS-Page at 125-128 kDa.  It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle.  It appears to form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation.  Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization.  Human AMPH is 695 amino acids (aa) in length.  It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands.  There is one known 108 kDa splice variant that shows a deletion of aa 425-466.  Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH. 

Alternate Names

AMPH, AMPH1

Entrez Gene IDs

273 (Human); 218038 (Mouse); 60668 (Rat)

Gene Symbol

AMPH

UniProt

P49418 (Human)

Additional Amphiphysin/AMPH Products

Product Documents for Human Amphiphysin/AMPH Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Amphiphysin/AMPH Antibody

For research use only

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