SW480 human colorectal adenocarcinoma cell line was stained with Rabbit anti-Human APCDD1 Monoclonal Antibody (Catalog # MAB10501, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Allophycoerythrin-conjugated Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human APCDD1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: SW480 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

References

Mazzoni, J. et al. (2017) Neuron. 96:1055.
Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
Shimomura, Y. (2010) Nature 464:1043.
Kandimalla, R. (2017) Oncogenesis 6:e308.
Yutaka Shimomura, et al. (2010) Nature 464:1043.
Sung-Gook CHO, (2017) Oncology Letters 14:4845.
Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Long Name

APC Down Regulated 1

Alternate Names

APC Down Regulated 1, DRAPC1, HYPT1

Entrez Gene IDs

147495 (Human); 494504 (Mouse); 689616 (Rat)

Gene Symbol

APCDD1

UniProt

Q8J025

Additional APCDD1 Products

Product Documents for Human APCDD1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human APCDD1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human APCDD1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10501

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all APCDD1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human APCDD1 Antibody

Detection of APCDD1 on SW480 Cell Line by Flow Cytometry.