Human APLP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3129
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human APLP-1
Gln34-Glu580
Accession # P51693
Gln34-Glu580
Accession # P51693
Specificity
Detects human APLP‑1 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse APLP-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human APLP-1 Antibody
APLP-1 in Human Brain.
APLP-1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human APLP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using out protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human and Mouse APLP-1 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP-1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Human APLP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human APLP-1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human APLP-1, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human APLP-1, see our available Western blot detection antibodies
Simple Western
25 µg/mL
Sample: Human brain (hippocampus) and mouse brain (hippocampus)
Sample: Human brain (hippocampus) and mouse brain (hippocampus)
Western Blot
0.1 µg/mL
Sample: Recombinant Human APLP-1
Sample: Recombinant Human APLP-1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APLP-1
Long Name
Amyloid-like Protein 1
Alternate Names
APLP1
Gene Symbol
APLP1
UniProt
Additional APLP-1 Products
Product Documents for Human APLP-1 Antibody
Product Specific Notices for Human APLP-1 Antibody
For research use only
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