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Human Apolipoprotein B/ApoB Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3556

R&D Systems, part of Bio-Techne
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BAF3556

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375

Specificity

Detects human Apolipoprotein B/ApoB in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Apolipoprotein B/ApoB Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Apolipoprotein B/ApoB
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Gene Symbol

APOB

UniProt

Additional Apolipoprotein B/ApoB Products

Product Documents for Human Apolipoprotein B/ApoB Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Apolipoprotein B/ApoB Biotinylated Antibody

For research use only

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