Human Apolipoprotein B100/ApoB100 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3260
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Functional Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ApoB100
Ile3374-Val3600
Accession # AAP72970
Ile3374-Val3600
Accession # AAP72970
Specificity
Detects human ApoB100 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Apolipoprotein B100/ApoB100 Antibody
Detection of Human Apolipoprotein B100/ApoB100 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Apolipoprotein B100/ApoB100 by Simple WesternTM.
Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 307 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.Applications for Human Apolipoprotein B100/ApoB100 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
1 µg/mL
Sample: Human plasma
Sample: Human plasma
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein B100/ApoB100
Alternate Names
apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4
Gene Symbol
APOB
UniProt
Additional Apolipoprotein B100/ApoB100 Products
Product Documents for Human Apolipoprotein B100/ApoB100 Antibody
Product Specific Notices for Human Apolipoprotein B100/ApoB100 Antibody
For research use only
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