Human Apolipoprotein C-II/ApoC2 Antibody

Apolipoprotein C-II/ApoC2 in THP-1 Human Cell Line.

Apolipoprotein C-II/ApoC2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Apolipoprotein C-II/ApoC2 in Human Liver.

Apolipoprotein C-II/ApoC2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Apolipoprotein C-II/ApoC2 ELISA Standard Curve.

Recombinant Human Apolipoprotein C-II/ApoC2 protein was serially diluted 2-fold and captured by Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (MAB4497) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB44971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).