| Specificity: |
Detects a synthetic peptide specific for human Aquaporin-4 around aa 315 in Direct ELISA.
Applications for Human Aquaporin 4/AQP4 Antibody
|
Recommended Concentration
|
Sample |
|
Immunohistochemistry |
Recommended Concentration
3-25 µg/mL |
Sample
Immersion fixed paraffin-embedded sections of human brain cortex |
|
Multiplex Immunofluorescence |
Recommended Concentration
25 µg/mL |
Sample
Immersion fixed paraffin-embedded sections of human brain cortex |
Scientific Data Examples for Human Aquaporin 4/AQP4 Antibody
|
|
Multiplex Immunofluorescence
Detection of AQP4 in Human Brain Cortex via seqIF™ staining on COMET™
AQP4 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Mouse Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11659) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder.
|
|
|
Immunohistochemistry
Detection of Aquaporin 4/AQP4 in Human Brain Cortex.
Aquaporin 4/AQP4 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human Aquaporin 4/AQP4 Monoclonal Antibody (Catalog # MAB11659) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of astrocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
|
Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
|
| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
|
| Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
|
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Reconstitution Calculator
Customer Reviews for Human Aquaporin 4/AQP4 Antibody
Have you used Human Aquaporin 4/AQP4 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Product Documents for Human Aquaporin 4/AQP4 Antibody
|
