Human Arylsulfatase A/ARSA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2485
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ARSA
Arg19-Ala507
Accession # AAH14210
Arg19-Ala507
Accession # AAH14210
Specificity
Detects human ARSA in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant mouse ARSA is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Arylsulfatase A/ARSA Antibody
Arylsulfatase A/ARSA in Human Liver.
Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Arylsulfatase A/ARSA Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)
Sample: Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)
Reviewed Applications
Read 1 review rated 4 using AF2485 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Arylsulfatase A/ARSA
As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.
References
- Lukatela, G. et al. (1998) Biochemistry 37:3654.
- Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Stein, C. et al. (1989) J. Biol. Chem. 264:1252.
Alternate Names
ARSA, Cerebroside 3-sulfatase
Gene Symbol
ARSA
UniProt
Additional Arylsulfatase A/ARSA Products
Product Documents for Human Arylsulfatase A/ARSA Antibody
Product Specific Notices for Human Arylsulfatase A/ARSA Antibody
For research use only
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