Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4494
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083
Ser29-Lys359 (Val151Ile)
Accession # Q02083
Specificity
Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse ASAHL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)
Sample: Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase
ASAHL (N-acylsphingosine-amidohydrolase-like; N acylethanolamine hydrolyzine acid amidase; also acid ceramidase like protein) is a 30 kDa member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30 31 kDa, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324 359, while another has a two aa substitution for aa 198 359. The human proform (aa 29 359) is 78% aa identical to mouse ASAHL proform.
References
Alternate Names
Acid ceramidase-like protein, ASAH-like protein, NAAA, Nacylethanolaminehydrolyzing Acid Amidase
Gene Symbol
NAAA
UniProt
Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products
- All Products for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase
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- ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Primary Antibodies
- ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Proteins and Enzymes
Product Documents for Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
Product Specific Notices for Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
For research use only
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