Human ASC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3805
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Knockout Validated, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ASC
Leu112-Ser195
Accession # Q9ULZ3
Leu112-Ser195
Accession # Q9ULZ3
Specificity
Detects human ASC in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ASC Antibody
Detection of Human ASC by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human ASC by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ASC at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human ASC Specificity by Using Knockout Cell Line.
Western blot shows lysates of MCF-7 human breast cancer parental cell line and ASC knockout MCF-7 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 23 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ASC Antibody
Application
Recommended Usage
Knockout Validated
ASC is
specifically detected in MCF‑7 human breast cancer parental cell line but is not detectable in ASC
knockout MCF‑7 cell line.
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line and U937 human histiocytic lymphoma cell line
Sample: MCF‑7 human breast cancer cell line and U937 human histiocytic lymphoma cell line
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line
Sample: MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASC
Apoptosis-associated speck-like protein containing a CARD (ASC) is also known as Target of Methylation-mediated Silencing (TMS-1) and PYD and CARD-domain containing protein (PYCARD). ASC is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli.
Long Name
Apoptosis-Associated Speck-Like Protein Containing A CARD
Alternate Names
CARD5, PYCARD, TMS1
Entrez Gene IDs
29108 (Human)
Gene Symbol
PYCARD
UniProt
Additional ASC Products
Product Documents for Human ASC Antibody
Product Specific Notices for Human ASC Antibody
For research use only
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