Human ATAD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9169

R&D Systems, part of Bio-Techne
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MAB9169-100
MAB9169-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 952947

View all ATAD2 Antibodies »

Product Summary for Human ATAD2 Antibody

Immunogen

Human ATAD2 synthetic peptide
Accession # Q6PL18

Specificity

Detects human ATAD2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ATAD2 Antibody

ATAD2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

ATAD2 in MCF‑7 Human Cell Line.

ATAD2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ATAD2 Monoclonal Antibody (Catalog # MAB9169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human ATAD2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATAD2

ATAD2/ANCCA/PRO2000 is an AAA (ATPases Associated with diverse cellular Activities) protein with two AAA domains and a bromodomain. ATAD2 is a coactivator in the regulatory process of ER alpha induction of target genes in breast cancer. Upon activation by estrogen, ATAD2 associates with ER alpha and ACTR and subsequently promotes expression of ER alpha target genes cyclin D1, c-myc and E2F1 in breast cancer cells.  ATAD2 also binds and hydrolyzes ATP, mediating hypermethylation of protein complexes. ATAD2 is reported to be a prognostic biomarker in endometrial, lung, ovarian and gastric cancers. The function of ATAD2 has also been reported in other carcinomas, including cervical, hepatocellular, colorectal and lung cancers.

Long Name

ATPase family, AAA domain containing 2

Alternate Names

ANCCA, CT137, PRO2000

Entrez Gene IDs

29028 (Human); 70472 (Mouse); 314993 (Rat)

Gene Symbol

ATAD2

UniProt

Q6PL18

Additional ATAD2 Products

Product Documents for Human ATAD2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ATAD2 Antibody

For research use only

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