Human ATG4A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4324
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A
Glu2-Val398
Accession # Q8WYN0
Glu2-Val398
Accession # Q8WYN0
Specificity
Detects human ATG4A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ATG4GB is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ATG4A Antibody
Detection of Human ATG4A by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG4A at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ATG4A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ATG4A, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ATG4A, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, MCF‑7 human breast cancer cell line, and human skeletal muscle tissue
Sample: HUVEC human umbilical vein endothelial cells, MCF‑7 human breast cancer cell line, and human skeletal muscle tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATG4A
ATG4A (Autophagy related protein 4 homolog A; also Autophagin 2) is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the Autophagin protein family which is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. There is a potential for multiple splice forms, namely isoform 1, isoform 2, and isoform 3. Human ATG4A shares 92% and 94% aa sequence identity with mouse and canine ATG4A, respectively.
Long Name
ATG4 Autophagy Related 4 Homolog A
Alternate Names
Apg4a, Atg4al, Autl2, Autophagin 2
Gene Symbol
ATG4A
UniProt
Additional ATG4A Products
Product Documents for Human ATG4A Antibody
Product Specific Notices for Human ATG4A Antibody
For research use only
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