Human ATG4B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5279
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 515803
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4B/Apg4b
Asp2-Leu393 (predicted)
Accession # Q9Y4P1
Asp2-Leu393 (predicted)
Accession # Q9Y4P1
Specificity
Detects human ATG4B/Apg4b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human ATG4B Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human ATG4B, see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human ATG4B, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human ATG4B
Sample: Recombinant Human ATG4B
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATG4B
Autophagy related protein 4 homolog B (ATG4B), also known as autophagin 1 and Apg4b, is a cytosolic cysteine protease that is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. Human ATG4B shares 98% and 95% amino acid sequence identity with chimpanzee and mouse ATG4B, respectively.
Long Name
ATG4 Autophagy Related 4 Homolog B
Alternate Names
Apg4B, AUTL1
Gene Symbol
ATG4B
UniProt
Additional ATG4B Products
Product Documents for Human ATG4B Antibody
Product Specific Notices for Human ATG4B Antibody
For research use only
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