Human ATR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4717
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ATR
Glu2405-Met2644
Accession # Q13535
Glu2405-Met2644
Accession # Q13535
Specificity
Detects human ATR in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ATR Antibody
Detection of Human ATR by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATR at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ATR in Human Ovarian Cancer Tissue.
ATR was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ATR Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATR
Long Name
Ataxia Telangiectasia and Rad 3-related
Alternate Names
FRP1, MEC1, SCKL1
Gene Symbol
ATR
UniProt
Additional ATR Products
Product Documents for Human ATR Antibody
Product Specific Notices for Human ATR Antibody
For research use only
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