Human Aurora A Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3295
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aurora A
Met1-Glu131
Accession # O14965
Met1-Glu131
Accession # O14965
Specificity
Detects human Aurora A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Aurora B and rhAurora C is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Aurora A Biotinylated Antibody
Aurora A in HeLa Human Cell Line.
Aurora A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Aurora A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3295) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to mitotic spindles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Aurora A Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Aurora A
Sample: Recombinant Human Aurora A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora A
Alternate Names
AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7
Gene Symbol
AURKA
UniProt
Additional Aurora A Products
Product Documents for Human Aurora A Biotinylated Antibody
Product Specific Notices for Human Aurora A Biotinylated Antibody
For research use only
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