Human Autoimmune Regulator/AIRE Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5936

R&D Systems, part of Bio-Techne
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AF5936
AF5936-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Autoimmune Regulator/AIRE Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AIRE
Ser476-Ser545
Accession # O43918

Specificity

Detects human AIRE in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Autoimmune Regulator/AIRE Antibody

Detection of Human Autoimmune Regulator/AIRE antibody by Western Blot.

Detection of Human Autoimmune Regulator/AIRE by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Human Autoimmune Regulator/AIRE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5936) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Autoimmune Regulator/AIRE at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Autoimmune Regulator/AIRE

AIRE (Autoimmune Regulator; also APECED) is a 58‑60 kDa member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545 amino acids (aa) in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa 1‑105), an NLS (aa 131‑133), a SAND domain (aa 181‑280), and two Zn-finger PHD domains (aa 296‑343 and 434‑475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa 437‑467, a 95 aa substitution for aa 1‑292, and a 75 aa substitution for aa 377‑545. Over aa 476‑545, human AIRE shares 71% aa identity with mouse AIRE.

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Gene Symbol

AIRE

UniProt

O43918 (Human)

Additional Autoimmune Regulator/AIRE Products

Product Documents for Human Autoimmune Regulator/AIRE Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Autoimmune Regulator/AIRE Antibody

For research use only

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