Human BACE-2 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4097
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 391017
Product Summary for Human BACE-2 Ectodomain Antibody
Immunogen
E. coli-derived recombinant human BACE-2
Ala21-Tyr473
Accession # Q9Y5Z0
Ala21-Tyr473
Accession # Q9Y5Z0
Specificity
Detects human BACE‑2 Ectodomain in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse BACE-2 is observed and no cross-reactivity with recombinant human (rh) BACE, rhCathepsin D, or rhCathepsin E is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human BACE-2 Ectodomain Antibody
BACE‑2 in Human Skin.
BACE-2 was detected in immersion fixed paraffin-embedded sections of human skin using Rat Anti-Human BACE-2 Ectodomain Monoclonal Antibody (Catalog # MAB4097) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BACE-2 Ectodomain Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin, placenta, and prostate
Sample: Immersion fixed paraffin-embedded sections of human skin, placenta, and prostate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-2
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 2
Alternate Names
BACE2
Gene Symbol
BACE2
UniProt
Additional BACE-2 Products
Product Documents for Human BACE-2 Ectodomain Antibody
Product Specific Notices for Human BACE-2 Ectodomain Antibody
For research use only
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