Human BACE-2 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4097
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE-2 Ectodomain
Gly63-Pro466
Accession # Q9Y5Z0
Gly63-Pro466
Accession # Q9Y5Z0
Specificity
Detects human and mouse BACE‑2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) BACE‑1, rhCathepsin D, and rhCathepsin E is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human BACE-2 Ectodomain Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE-2, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE-2, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample:
Sample:
Recombinant Human BACE‑2
Recombinant Mouse BACE-2 (Catalog # 2977-BA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-2
BACE-2 ( beta-site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a beta-secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa’s 329‑378, while a second shows an 18 aa substitution for aa’s 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE‑2.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 2
Alternate Names
BACE2
Gene Symbol
BACE2
UniProt
Additional BACE-2 Products
Product Documents for Human BACE-2 Ectodomain Antibody
Product Specific Notices for Human BACE-2 Ectodomain Antibody
For research use only
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