Human BANK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8005

R&D Systems, part of Bio-Techne
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AF8005
AF8005-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all BANK1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BANK1
Ser480-His785 (Cys650Arg)
Accession # Q8NDB2

Specificity

Detects human BANK1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human BANK1 Antibody

Detection of Human BANK1 antibody by Western Blot.

Detection of Human BANK1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BANK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BANK1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human BANK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BANK1

BANK1 (B cell scaffold protein with ANKyrin repeats) is a cytoplasmic scaffold protein containing ankyrin repeats. Although its predicted MW is 85 kDa, due to its highly acidic nature, it runs anomalously at 97-105 kDa in SDS-PAGE. It is expressed in select cell types, including mature B cells and pancreatic islet beta‑cells. In B cells, following BCR activation, IP3 is generated and BANK1 is phosphorylated by BCR-associated Syk (Spleen Tyr kinase). At this point, phosphorylated BANK1 subsequently interacts with both cytosolic Lyn (Lck/Yes-related Novel tyrosine kinase) and IP3R, leading to an IP3 receptor highly sensitive to IP3. Increased cellular IP3 binding to IP3R stimulates the release of calcium from intracellular stores. BANK1 is also posited to participate in the regulation of IgM production, its role in this case being that of a negative modulator of secretion. Human BANK1 is 785 amino acids (aa) in length. It contains an IP3R interaction region (aa 1-154), followed by a DBB domain (aa 200-327) that is involved in dimerization, and two consecutive ANK repeats (aa 345-408). There are at least three isoform variants. One utilizes an alternative start site at Met31, a second contains an eight aa substitution for aa 1-23, and a third shows a deletion of aa 24-156. BANK1 is known to form homo- and heterodimers with its isoforms, and a Arg61 to His61 transition is reported to be correlated with reduced homooligomer formation. Over aa 480-785, human BANK1 shares 73% aa sequence identity with mouse BANK1.

Long Name

B-cell Scaffold Protein with Ankyrin Repeats 1

Alternate Names

BANKFLJ20706B-cell scaffold protein with ankyrin repeats, B-cell scaffold protein with ankyrin repeats 1, FLJ34204

Entrez Gene IDs

55024 (Human); 242248 (Mouse); 365948 (Rat)

Gene Symbol

BANK1

UniProt

Q8NDB2

Additional BANK1 Products

Product Documents for Human BANK1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BANK1 Antibody

For research use only

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