U266 human myeloma cell line was stained with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB107621, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of BCMA/TNFRSF17 in RPMI8226 Human Cell Line by Flow Cytometry

RPMI8226 human myeloma cell line was stained with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB107621, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

BCMA/TNFRSF17 in U266 Human Cell Line .

BCMA/TNFRSF17 was detected in immersion fixed U266 human myeloma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB107621) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human BCMA/TNFRSF17 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RPMI8226 and U266 human myeloma cell lines

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U266 human myeloma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q6PE46

Additional BCMA/TNFRSF17 Products

Product Documents for Human BCMA/TNFRSF17 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human BCMA/TNFRSF17 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human BCMA/TNFRSF17 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB107621

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BCMA/TNFRSF17 Antibody

Detection of BCMA/TNFRSF17 in U266 Human Cell Line by Flow Cytometry.