Human BCR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5129
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BCR
Lys174-Asp331
Accession # P11274
Lys174-Asp331
Accession # P11274
Specificity
Detects human BCR in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human BCR Antibody
Detection of Human BCR by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human BCR Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line
Sample: HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCR
BCR (Breakpoint cluster region protein) is a novel 155‑165 kDa Ser/Thr protein kinase. It is found in fibroblasts and hematopoietic cells, interacts with 14-3-3, and serves as a natural inhibitor of the growth factor-associated c-Abl nonreceptor protein tyrosine kinase. Human BCR is 1271 amino acids (aa) in length. It contains a coiled-coil dimerization motif (aa 28‑68), a Ser/Thr kinase region (aa 176‑426), one DH domain (aa 498‑691) and a Rho-Gap region (aa 1054‑1248). The N-terminal aa 1‑902 and 1‑927 are known to contribute to the BCR‑ABL oncoprotein. BCR likely forms homodimers and 650 kDa homotetramers. An alternate start site exists at Met498, while other isoforms show a deletion of aa 961‑1004, plus a 61 and 37 aa substitution for aa 1‑488. Over aa 1‑426, human BCR is 89% aa identical to mouse BCR.
Long Name
Breakpoint Cluster Region
Alternate Names
ALL, PHL
Gene Symbol
BCR
UniProt
Additional BCR Products
Product Documents for Human BCR Antibody
Product Specific Notices for Human BCR Antibody
For research use only
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