Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB85602

Human B3GAT1 MAb (Clone 1021209)
R&D Systems, part of Bio-Techne
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MAB85602-100
MAB85602-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1021209

View all beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human beta‑1,3‑Glucuronyltransferase 1/B3GAT1
His25-Ile334
Accession # Q9P2W7

Specificity

Detects human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Detection of Human beta-1,3-Glucuronyltransferase 1/B3GAT1 antibody by Western Blot.

Detection of Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 by Western Blot.

Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta-1,3-Glucuronyltransferase 1/B3GAT1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat beta-1,3-Glucuronyltransferase 1/B3GAT1 antibody by Simple WesternTM.

Detection of Mouse and Rat beta‑1,3‑Glucuronyltransferase 1/B3GAT1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain tissue and rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for beta‑1,3‑Glucuronyltransferase 1/B3GAT1 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
beta-1,3-Glucuronyltransferase 1/B3GAT1 antibody in Human Brain by Immunohistochemistry (IHC-P).

beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in Human Brain.

beta-1,3-Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Simple Western

20 µg/mL
Sample: Mouse brain tissue and Rat brain tissue

Western Blot

2 µg/mL
Sample: Human cerebellum tissue

Reviewed Applications

Read 1 review rated 5 using MAB85602 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta1-3Gal beta1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

  1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
  2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
  3. Kakuda, S. et al. (2005) Glycobiology 2:203.
  4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
  5. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Alternate Names

CD57, GLCATP, GLCUATP, HNK1, LEU7

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Gene Symbol

B3GAT1

UniProt

Q9P2W7

Additional beta-1,3-Glucuronyltransferase 1/B3GAT1 Products

Product Documents for Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Product Datasheets

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Product Specific Notices for Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

For research use only

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