Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3960

R&D Systems, part of Bio-Techne
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MAB3960
MAB3960-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 485327

View all Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97

Specificity

Detects human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human B4GALT1 is observed

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 (Catalog # 3960-GT)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

beta1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta1,3‑N‑acetylglucosaminyltransferases, that includes at least eight members (1‑5).  All of them are type II Golgi resident transmembrane proteins and have high homology to the beta1,3-galactosyltransferase family. beta1,3‑N‑acetylglucosaminyltransferases 2 or beta3GNT2 prefers the substrate lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains (6).

References

  1. Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
  2. Kataoka, K. and Huh, N.-H. (2002) Biochem. Biophys. Res. Commun. 294:843.
  3. Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.

  4. Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.

  5. Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.

  6. Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Alternate Names

B3Galt6, Beta3gnt1

Entrez Gene IDs

10678 (Human); 53625 (Mouse)

Gene Symbol

B3GNT2

UniProt

Q9NY97

Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products

Product Documents for Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

For research use only

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