HEK293 human embryonic kidney cell line transfected with either (A) human beta-3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta-3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

beta‑3 Adrenergic R/ADRB3 in Human Adipocytes.

beta-3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta-3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human beta-3 Adrenergic R/ADRB3 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human beta-3 Adrenergic R/ADRB3 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human adipocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-3 Adrenergic R/ADRB3

ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).

Long Name

Beta-3 Adrenergic Receptor

Alternate Names

ADRB3, ADRB3R, B3AR, beta-3 AdrenergicR, beta3 AdrenergicR

Entrez Gene IDs

155 (Human); 11556 (Mouse); 25645 (Rat)

Gene Symbol

ADRB3

UniProt

P13945

Additional beta-3 Adrenergic R/ADRB3 Products

Product Documents for Human beta-3 Adrenergic R/ADRB3 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human beta-3 Adrenergic R/ADRB3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human beta-3 Adrenergic R/ADRB3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9419

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all beta-3 Adrenergic R/ADRB3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human beta-3 Adrenergic R/ADRB3 Antibody

Detection of beta‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry.