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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 196624

Product Specifications

Immunogen

E. coli-derived recombinant human beta-Catenin
Ala2-Leu781
Accession # P35222

Specificity

Detects human, mouse, and rat beta-Catenin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human beta-Catenin APC-conjugated Antibody

Detection of  beta-Catenin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of beta‑Catenin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta-Catenin APC-conjugated Monoclonal Antibody (Catalog # IC13292A, filled histogram) or isotype control antibody (Catalog # IC003A, open histo-gram). To facilitate intracellular staining, cells were fixed with 4% para-formaldehyde and permeabilized with Methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human beta-Catenin APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with 4% paraformaldehyde and permeabilized with Methanol.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-Catenin

beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Gene Symbol

CTNNB1

UniProt

Additional beta-Catenin Products

Product Documents for Human beta-Catenin APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human beta-Catenin APC-conjugated Antibody

For research use only

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