Human BIM Long (BIM L) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1325
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BIML Long (BIM L)
Ala2-Arg120
Accession # NP_006529
Ala2-Arg120
Accession # NP_006529
Specificity
Detects human BIML in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human BIM Long (BIM L) Antibody
BIMLin Human Spleen.
BIMLwas detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human BIMLLong (BIM L) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and perinuclear area in monocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BIM Long (BIM L) Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF1325 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BIM Long (BIM L)
BIML (also known as Bod) is one of several splice variants of BIM, a pro-apoptotic protein belonging to the BH3 domain-only subgroup of Bcl-2 family members. BIML is thought to promote apoptosis by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria. BIML is normally sequestered in an inactive conformation from anti-apoptotic Bcl-2 family members through binding to the microtubule-associated dynein motor complex.Certain apoptotic stimuli release BIML from microtubules to neutralize anti-apoptotic Bcl-2 family members, allowing for the initiation of apoptosis.
References
- O'Connor, L. et al. (1998) EMBO J. 17:384.
- Puthalakath, H. et al. (1999) Mol. Cell. 3:287.
- Miyashita, T. (2001) FEBS Letters 509:135.
- Strasser, A. (2000) Ann N Y Acad Sci. 917:541.
- Marani, M. et al. (2002) Mol. Cell. Biol. 22:3577.
Long Name
Bcl-2 Interacting Mediator of Cell Death
Alternate Names
BCL2L11
Gene Symbol
BCL2L11
UniProt
Additional BIM Long (BIM L) Products
Product Documents for Human BIM Long (BIM L) Antibody
Product Specific Notices for Human BIM Long (BIM L) Antibody
For research use only
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