Human BMI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB33342
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 384509
Product Specifications
Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Asp96-Gly326
Accession # P35226
Specificity
Detects human BMI-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human BMI-1 Antibody
BMI-1 in Human Breast Cancer Tissue.
BMI-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
BMI‑1 in Human Breast.
BMI-1 was detected in immersion fixed paraffin-embedded sections of normal human breast using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BMI-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of normal human breast and human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of normal human breast and human breast cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB33342 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMI-1
BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 is 99%, 97%, 99% and 99% aa identical to bovine, mouse, feline and canine BMI-1, respectively.
Long Name
B Lymphoma Mo-MLV Insertion Region 1
Alternate Names
BMI1, PCGF4, RNF51
Gene Symbol
BMI1
UniProt
Additional BMI-1 Products
Product Documents for Human BMI-1 Antibody
Product Specific Notices for Human BMI-1 Antibody
For research use only
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