Human BMP-3b/GDF-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1543
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Gln369-Arg478
Accession # Q5VSQ8
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human BMP-3b/GDF-10 Antibody
Detection of BMP-3b/GDF-10 in Human Prostate Cancer.
BMP-3b/GDF-10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Goat Anti-Human BMP-3b/GDF-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1543) at 10 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BMP-3b/GDF-10 Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Western Blot
Sample: Recombinant Human BMP-3b/GDF-10 (Catalog # 1543-BP)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-3b/GDF-10
BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2‑4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).
References
- Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
- Cunningham, N.S. et al. (1995) Growth Factors 12:99.
- Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
- Zhao, R. et al. (1999) Dev. Biol. 212:68.
- Hino, J. et al. (1999) Biochem .Biophys. Res. Comm. 256:419.
- Kaihara, S. et al. (2003) Life Sci. 72:1683.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional BMP-3b/GDF-10 Products
Product Documents for Human BMP-3b/GDF-10 Antibody
Product Specific Notices for Human BMP-3b/GDF-10 Antibody
For research use only