Human BMPR-II Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF811
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-II
Ala26-Ile151
Accession # Q13873
Ala26-Ile151
Accession # Q13873
Specificity
Detects human BMPR‑II in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) BMPR-IA and rhBMPR-1B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human BMPR-II Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: PC-3 human prostate carcinoma cell line
Sample: PC-3 human prostate carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Western Blot
0.1 µg/mL
Sample: Recombinant Human BMPR-II Fc Chimera (Catalog # 811-BR)
Sample: Recombinant Human BMPR-II Fc Chimera (Catalog # 811-BR)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-II
Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signl transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4 and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity.
References
- Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
- Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
- Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Long Name
Bone Morphogenetic Protein Receptor II
Alternate Names
BMPR2, BMPRII
Gene Symbol
BMPR2
UniProt
Additional BMPR-II Products
Product Documents for Human BMPR-II Biotinylated Antibody
Product Specific Notices for Human BMPR-II Biotinylated Antibody
For research use only
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