Human BTK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5807
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 566928
Product Specifications
Immunogen
E. coli-derived recombinant human BTK
Glu280-Val377
Accession # Q06187
Glu280-Val377
Accession # Q06187
Specificity
Detects endogenous human BTK in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human BTK Antibody
Detection of Human BTK by Western Blot.
Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.05 µg/ml of Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BTK at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
BTK in Raji Human Cell Line.
BTK was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human BTK by Simple WesternTM.
Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for BTK at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human BTK Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Simple Western
10 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line
Western Blot
0.05 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Reviewed Applications
Read 1 review rated 5 using MAB5807 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTK
Long Name
Bruton Agammaglobulinemia Tyrosine Kinase
Alternate Names
AGMX1, IMD1, PSCTK1, XLA
Entrez Gene IDs
695 (Human)
Gene Symbol
BTK
UniProt
Additional BTK Products
Product Documents for Human BTK Antibody
Product Specific Notices for Human BTK Antibody
For research use only
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