Human BTLA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3354
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 330104
Product Summary for Human BTLA Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTLA
Ile25-Ser150
Accession # AAP44003
Ile25-Ser150
Accession # AAP44003
Specificity
Detects human BTLA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse BTLA or recombinant human HVEM is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human BTLA Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BTLA
Sample: Recombinant Human BTLA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTLA
B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
UniProt
Additional BTLA Products
Product Documents for Human BTLA Antibody
Product Specific Notices for Human BTLA Antibody
For research use only
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