Human BTLA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3354
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 330104
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTLA
Ile25-Ser150
Accession # AAP44003
Ile25-Ser150
Accession # AAP44003
Specificity
Detects human BTLA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse BTLA or recombinant human HVEM is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human BTLA Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BTLA
Sample: Recombinant Human BTLA
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTLA
B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
UniProt
Additional BTLA Products
Product Documents for Human BTLA Antibody
Product Specific Notices for Human BTLA Antibody
For research use only
Loading...
Loading...
Loading...
Loading...