Human BTLA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3354
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTLA
Ile25-Ser150
Accession # AAP44003
Ile25-Ser150
Accession # AAP44003
Specificity
Detects human BTLA in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human BTLA Antibody
Detection of Human BTLA by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human BTLA. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human BTLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BTLA at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human BTLA Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood lymphocytes
Sample: Human whole blood lymphocytes
Western Blot
0.5 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human BTLA
Sample: HEK293T human embryonic kidney cell line transfected with human BTLA
Reviewed Applications
Read 1 review rated 5 using AF3354 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTLA
B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one
V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
UniProt
Additional BTLA Products
Product Documents for Human BTLA Antibody
Product Specific Notices for Human BTLA Antibody
For research use only
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