Human BTN1A1/Butyrophilin Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB84671U
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2151B
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTN1A1/Butyrophilin
Ala27-Arg242
Accession # Q13410
Ala27-Arg242
Accession # Q13410
Specificity
Detects human BTN1A1/Butyrophilin in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human BTN1A1 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human BTN1A1 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BTN1A1/Butyrophilin
Butyrophilin 1A1 (also called BTN1A1), a 55kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma delta T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of human BTN1A1 shares 68% aa sequence identity with both mouse and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.
References
- Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
- Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
- Vorback, C. et al. (2002) Genes Dev. 16:3223.
- Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084.
- Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385.
- Sandstrom, A. et al. (2014) Immunity 40:490.
- Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559.
- Smith, I.A. et al. (2010) J.Immunol. 184:3514.
- Mana, P. et al. (2004) J.Immunol. 16:489.
Alternate Names
BTN, BTN1A1
Gene Symbol
BTN1A1
UniProt
Additional BTN1A1/Butyrophilin Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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