Human BTN3A1/2/3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7136
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gln30-Trp248
Accession # P78410
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human BTN3A1/2/3 Antibody
Detection of Human BTN3A1/2/3 by Western Blot.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BTN3A1/2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN3A1/2/3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human BTN3A1/2/3 Antibody
Western Blot
Sample: SK‑Mel‑28 human malignant melanoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTN3A1/2/3
BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region (aa 30-248) with one V-type Ig-like domain (aa 30-134), and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. One possesses an alternative start site 43 aa upstream of the standard site, a second contains a five aa substitution for aa 1-28, and a third shows a 24 aa substitution for aa 307-334. A rodent counterpart to BTN3A2 has not been reported. Human BTN3A1 (Butyrophilin subfamily 3 member A1; also BTF5) over aa 30-248 shares 95.4% aa identity with human NTN3A2.
Long Name
Alternate Names
UniProt
Additional BTN3A1/2/3 Products
Product Documents for Human BTN3A1/2/3 Antibody
Product Specific Notices for Human BTN3A1/2/3 Antibody
For research use only