SW480 human cell line was stained with Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Human C10orf99 ELISA Standard Curve.

Recombinant Human C10orf99 protein was serially diluted 2-fold and captured by Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB10416) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of C10orf99 in T84 cells by Flow Cytometry.

T84 human epithelial carcinoma cells treated with 5 μg/ml Brefeldin A 1231/5 for 5 hours were stained with Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human C10orf99 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB10416).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: SW40 human cell line and MDA-MB-231 cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C10orf99

Human Chromosome 10 open reading frame 99 is a 9.2 KDa protein encoded by the C10orf99 gene. C10orf99 has been described as a potential cytokine and a ligand Sushi Domain Containing 2 (SUSD2), inducing SUSD2 internalization upon binding. It has also been proposed to be a chemotactic factor that mediates lymphocytes recruitment to epithelia through binding and activation of the G-protein coupled receptor GPR15.

Long Name

Chromosome 10 Open Reading Frame 99

Alternate Names

AP-57, AP57, GPR15L

Entrez Gene IDs

387695 (Human); 70045 (Mouse)

Gene Symbol

C10ORF99

Additional C10orf99 Products

Product Documents for Human C10orf99 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human C10orf99 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human C10orf99 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB104161

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all C10orf99 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human C10orf99 Antibody

Detection of C10orf99 in SW480 human cell line.