Human Cadherin-6/KCAD Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2715N

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 427909

View all Cadherin-6/KCAD Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cadherin‑6/KCAD
Ser54-Ala615
Accession # P55285

Specificity

Detects human Cadherin‑6/KCAD in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, -11, -12, -13, -17, rhECAD, rhMCAD, rhNCAD, rhRCAD, rhPCAD, or rhVECAD is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cadherin-6/KCAD Alexa Fluor® 700-conjugated Antibody

Detection of Cadherin-6/KCAD antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

Detection of Cadherin‑6/KCAD in MG‑63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human Cadherin-6/KCAD Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2715N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Applications for Human Cadherin-6/KCAD Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: MG‑63 human osteosarcoma cell line stained in buffer containing Ca2+ and Mg2+
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Cadherin-6/KCAD

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium‑dependent, cell-to-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin‑6, also known as KCAD or K-cadherin, is a classical cadherin of 110‑120 kD that has at least one full length and two alternate splice forms ranging in size from 105‑120 kDa (3). Human Cadherin-6 is synthesized as a 790 amino acid (aa) type I transmembrane glycoprotein that contains an 18 aa signal peptide, a 35 aa prosequence, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (4, 5). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium‑dependent cell-to-cell adhesion through their Ca++-binding repeats (2). Due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat, Cadherin-6 can be further classified as a type II classical cadherin (4). One Cadherin‑6 splice variant (termed 6/2) shows a 9 aa substitution for the 94 aa that span residues 283 to 376 of the full-length extracellular region (3). A second splice variant shows a 36 aa substitution for the C-terminal 163 aa of the transmembrane and cytoplasmic region (6). The extracellular region of human Cadherin‑6 is 98% aa identical to rat Cadherin‑6 extracellular region, plus 60% and 58% aa identical to the extracellular regions of human cadherin 8 and 11, respectively. Cadherin-6 has high expression in kidney, brain, and cerebellum, and low expression in lung, pancreas, gastric mucosa, and cytotrophoblasts (4, 5, 7, 8, 9). Cadherin-6 is also found in renal, lung, and ovarian carcinoma (7, 10). As a classic cadherin, Cadherin-6 will form homodimers and promote intercellular adhesion with itself and possibly, cadherin-9 and -14 (4, 11).

References

  1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
  6. GenBank Accession # P55285.
  7. Xiang Y.Y. et al. (1994) Cancer Res. 54:3034.
  8. Marthiens V. et al. (2002) Mol. Cell Neurosci. 20:458.
  9. MacCalman C.D. et al. (1998) Am J Reprod. Immunol. 39:96.
  10. Sella, G.C. et al. (2001) Cancer Res. 61:6977.
  11. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.

Alternate Names

Cadherin6, CDH6, K-Cadherin

Entrez Gene IDs

1004 (Human)

Gene Symbol

CDH6

UniProt

P55285

Additional Cadherin-6/KCAD Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cadherin-6/KCAD Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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