MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Calcitonin R Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Calcitonin R knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Detection of Human Calcitonin R by Immunocytochemistry/Immunofluorescence

Effects of AA and DHA on actin ring formation and CTR expression.Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μM) were added from day 3 in differentiating osteoclasts and from the onset of resorption (day 12–14) in mature osteoclasts. After 3 weeks of culture, cells were fixed and stained with Hoechst (blue) for nuclei, phalloidin (red) for actin rings, and anti-CTR antibody (green). The results are representative of three independent experiments conducted in triplicate. Scale bar = 200 μm. Diff Ocl—differentiating osteoclasts. Mat Ocl—mature osteoclasts. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25867515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Calcitonin R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: MCF-7 human breast cancer cell line

Knockout Validated

Calcitonin R is specifically detected in MCF-7 human breast cancer parental cell line but is not detectable in Calcitonin R knockout MCF-7 cell line.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcitonin R

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.

Long Name

Calcitonin Receptor

Alternate Names

CalcitoninR, CALCR, CT-R

Entrez Gene IDs

799 (Human); 12311 (Mouse); 116506 (Rat)

Gene Symbol

CALCR

UniProt

NP_001733

Additional Calcitonin R Products

Product Documents for Human Calcitonin R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Calcitonin R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Calcitonin R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4614

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Calcitonin R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Calcitonin R Antibody

Detection of Calcitonin R in MCF‑7 Human Cell Line by Flow Cytometry.