Human Caldesmon/CALD1 Antibody

Detection of Human Caldesmon/CALD1 by Western Blot.

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caldesmon/CALD1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caldesmon/CALD1 in WI‑38 Human Cell Line.

Caldesmon/CALD1 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Caldesmon/CALD1 in Endometrial Carcinoma.

Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of endometrial carcinoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.